Tg(Msx2-En1)86Alj
Transgene Detail

Summary

• Symbol: Tg(Msx2-En1)86Alj
• Name: transgene insertion 86, Alexandra L Joyner
• MGI ID: MGI:4834774
• Synonyms: Msx2-En1
• Transgene: Tg(Msx2-En1)86Alj Location: unknown
• Alliance: Tg(Msx2-En1)86Alj page

Transgene origin

• Strain of Origin: Swiss Webster

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Msx2-En1)86Alj expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	En1 (MGI:95389)

• Mutation details: A transgenic construct was generated with the En1 cDNA driven by a 520 bp apical ectodermal ridge (AER)-specific Msx2 promoter. The purified construct was injected into Swiss Webster zygotes. Six founders were obtained. Founder 86 showed a dramatic limb phenotye, while 2 others (25, 95) appeared normal, but offspring had similar phenotypes (line 25; 100% of pups and line 95; 60% of pups) seen in line 86 (100% of pups). Two founders did not transmit the transgene, while the other transmitted the transgene but offspring showed no phenotype. Line 86 was studied in detail. (J:62572)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:62572 Kimmel RA, et al., Two lineage boundaries coordinate vertebrate apical ectodermal ridge formation. Genes Dev. 2000 Jun 1;14(11):1377-89
• All: 1 reference(s)