Igh-7^tm1Lcwu
Targeted Allele Detail

Summary

• Symbol: Igh-7^tm1Lcwu
• Name: immunoglobulin heavy chain 7 (heavy chain of IgE); targeted mutation 1, Lawren C Wu
• MGI ID: MGI:4461407
• Gene: Igh-7 Location: Chr12:113228142-113241336 bp Genetic Position: Chr12, Syntenic
• Alliance: Igh-7^tm1Lcwu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:161452
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Humanized sequence, Inserted expressed sequence) Mutation: Insertion Mutation details: DNA sequence encoding the human M1' segment was inserted immediately following the splice acceptor site of the mouse IgE M1 exon. In addition, an IRES element followed by a GFP construct modified to encode an F64A amino acid change and then a floxed neomycin selection cassette were all inserted downstream of the mouse IgE M2 exon. The selection cassette was subsequently excised by transient in vitro cre expression. (J:161452)

Generation of the Igh-7^tm1Lcwu allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh-7 Mutation: 1 strain or line available

References

• Original: J:161452 Brightbill HD, et al., Antibodies specific for a segment of human membrane IgE deplete IgE-producing B cells in humanized mice. J Clin Invest. 2010 Jun 1;120(6):2218-29
• All: 3 reference(s)