Dnmt3b^tm2.1Ics
Targeted Allele Detail

Summary

• Symbol: Dnmt3b^tm2.1Ics
• Name: DNA methyltransferase 3B; targeted mutation 2.1, Mouse Clinical Institute
• MGI ID: MGI:4460908
• Synonyms: Dmnt3b^D.823G, Dnmt3b^mEx24
• Gene: Dnmt3b Location: Chr2:153491370-153529650 bp, + strand Genetic Position: Chr2, 75.79 cM, cytoband A2-A3
• Alliance: Dnmt3b^tm2.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:160567
• Parent Cell Line: P1 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: An A to G transition in exon 24 results in the amino acid substitution of glycine for aspartic acid at position 823 (D823G). A floxed neo cassette inserted upstream of the modified exon 24 was removed by cre mediated recombination. (J:160567)

Expression

In Mice Carrying this Mutation:	42 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3b Mutation: 50 strains or lines available

References

• Original: J:160567 Velasco G, et al., Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues. Proc Natl Acad Sci U S A. 2010 May 18;107(20):9281-6
• All: 2 reference(s)