Ascl1tm1.1(cre/ERT2)Jejo
Targeted Allele Detail
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Symbol: |
Ascl1tm1.1(cre/ERT2)Jejo |
Name: |
achaete-scute family bHLH transcription factor 1; targeted mutation 1.1, Jane Johnson |
MGI ID: |
MGI:4452601 |
Synonyms: |
Ascl1-CreER, Ascl1CreERT2 |
Gene: |
Ascl1 Location: Chr10:87326681-87329522 bp, - strand Genetic Position: Chr10, 43.64 cM
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Alliance: |
Ascl1tm1.1(cre/ERT2)Jejo page
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Germline Transmission: |
Earliest citation of germline transmission:
J:171439
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Parent Cell Line: |
W4 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutations: |
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Insertion, Intragenic deletion
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Ascl1tm1.1(cre/ERT2)Jejo expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Ascl1 (MGI:96919) |
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Mutation details: A targeting vector was designed to replace the entire coding region of the achaete-scute complex homolog 1 (Ascl1 or Mash1) with a CreERT2 fusion protein and a frt-flanked neomycin (neo) selection cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked sequence. These mice were then backcrossed at least 5 generations to and outbred ICR strain generate a colony of Ascl1-CreERT2 mice.
(J:171439)
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Generation of the Ascl1tm1.1(cre/ERT2)Jejo allele |
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Ascl1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:171439 Kim EJ, et al., Ascl1 (mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain. PLoS One. 2011;6(3):e18472 |
All: |
65 reference(s) |
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