Klf3^{tm1(cre/ERT2)Pzg}
Targeted Allele Detail

Summary

• Symbol: Klf3^{tm1(cre/ERT2)Pzg}
• Name: Kruppel-like transcription factor 3 (basic); targeted mutation 1, Pumin Zhang
• MGI ID: MGI:4441459
• Synonyms: Klf3-GCE, Klf3-GFP-Cre-ERT2, Klf3^{tm1(cre/ERT2)Zhang}
• Gene: Klf3 Location: Chr5:64960864-64990246 bp, + strand Genetic Position: Chr5, 33.49 cM
• Alliance: Klf3^{tm1(cre/ERT2)Pzg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159099
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Klf3^{tm1(cre/ERT2)Pzg} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Klf3 (MGI:1342773)

• Mutation details: Mice with this mutation have a tamoxifen-inducible Cre-mediated recombination system and, independently, express green fluorescent protein (GFP) from the promoter of the endogenous gene. A GFP-Cre/ERT2 cassette (GCE) with a polyadenylation signal, followed by a frt site-flanked kanamycin and neomycin resistance cassette, was inserted in the first coding exon of the endogenous gene immediately following the ATG translation initiation codon. Weak green fluorescence is detected in the back, limbs and kidneys of E15.5 heterozygous embryos. Following tamoxifen induction of Cre recombinase activity in E15.5 embryos bearing this allele and a Cre-inducible lacZ reporter allele, beta-galactosidase is detected by X-gal staining in the Kif3a domain of the urogenital system. (J:159099)
• Inheritance: Not Specified

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Klf3 (mouse) Summary of all recombinase alleles driven by Klf3. Alliance mouse Klf3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Klf3 Mutation: 80 strains or lines available

References

• Original: J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010
• All: 3 reference(s)