Prox1^tm4.1Gco
Targeted Allele Detail

Summary

• Symbol: Prox1^tm4.1Gco
• Name: prospero homeobox 1; targeted mutation 4.1, Guillermo Oliver
• MGI ID: MGI:4441290
• Synonyms: Prox1^NRMut
• Gene: Prox1 Location: Chr1:189850232-189902911 bp, - strand Genetic Position: Chr1, 95.85 cM
• Alliance: Prox1^tm4.1Gco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158959
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector containing a 2.5 kb fragment upstream of the ATG of mouse Prox1, a 1.5 kb fragment containing a fusion of synthetic avitag-encoding sequence (avitag is a 17-amino-acid peptide (MSGLNDIFEAQKIEWHE) that can be biotinylated by the BirA biotin ligase, and therefore can be used for immunoprecipitation of tagged proteins using streptavidin), and Prox1 exon 1 carrying mutations in the sequence coding for the nuclear hormone-binding site (LRKLL->ARKAL, ISQLL->ASQAL), was generated. The targeting vector also contained a loxP-flanked neomycin selection cassette for positive selection and a DTA cassette for negative selection. Homologous recombination of the Prox1 locus was carried out in W9.5 ES cells. Correctly targeted embryonic stem cells were used for the generation of chimeric mice. The neomycin cassette in the F1 Prox1 mutants was removed by crossing with a Vav-iCre transgenic line. (J:158959)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prox1 Mutation: 43 strains or lines available

References

• Original: J:158959 Srinivasan RS, et al., The nuclear hormone receptor Coup-TFII is required for the initiation and early maintenance of Prox1 expression in lymphatic endothelial cells. Genes Dev. 2010 Apr 1;24(7):696-707
• All: 2 reference(s)