Tie1^tm1Scba
Targeted Allele Detail

Summary

• Symbol: Tie1^tm1Scba
• Name: tyrosine kinase with immunoglobulin-like and EGF-like domains 1; targeted mutation 1, Scott Baldwin
• MGI ID: MGI:4441286
• Synonyms: Tie1^neo
• Gene: Tie1 Location: Chr4:118328388-118347046 bp, - strand Genetic Position: Chr4, 54.67 cM
• Alliance: Tie1^tm1Scba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158668
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 1 and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 1. Western blot analysis confirmed a 76% reduction in protein expression in the lungs of E18.5 homozygous mice. This is a hypomorphic allele. (J:158668)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tie1 Mutation: 67 strains or lines available

References

• Original: J:158668 Qu X, et al., Abnormal embryonic lymphatic vessel development in Tie1 hypomorphic mice. Development. 2010 Apr;137(8):1285-95
• All: 7 reference(s)