Tlcd4^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Tlcd4^{tm1a(EUCOMM)Hmgu}
• Name: TLC domain containing 4; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4435938
• Synonyms: TMEM56^-
• Gene: Tlcd4 Location: Chr3:120995657-121076756 bp, - strand Genetic Position: Chr3, 52.94 cM, cytoband G3
• Alliance: Tlcd4^{tm1a(EUCOMM)Hmgu} page
• IMPC: Tlcd4 gene page

Mutation origin

• Mutant Cell Lines: HEPD0529_2_B02, HEPD0529_2_B04, HEPD0529_2_C02, HEPD0529_2_C03, HEPD0529_2_E02, HEPD0529_2_E04, HEPD0529_2_G03
• Germline Transmission: Earliest citation of germline transmission: J:385397
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 121021659 of Chromosome 3 upstream of the critical exons 5 and 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 121023041. Exons 5 and 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. qRT-PCR analysis using primer pairs covering exons 7 and 8 revealed residual expression of approximately 20-25% of wild-type levels in liver samples from adult homozygous mutant mice. Western blot analysis using an antibody specific for the C-terminal region confirmed the absence of full-length protein in homozygous mutants. No shorter protein variants corresponding to exons 7-8 were detected. This allele represents a strong hypomorphic, and likely null, mutation. (J:157065, J:385397)

Expression

In Mice Carrying this Mutation:

52 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tlcd4 Mutation: 20 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)