Gatad2b^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gatad2b^{tm1a(EUCOMM)Hmgu}
• Name: GATA zinc finger domain containing 2B; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4434913
• Synonyms: Gatad2b^stop
• Gene: Gatad2b Location: Chr3:90200488-90270712 bp, + strand Genetic Position: Chr3, 39.21 cM
• Alliance: Gatad2b^{tm1a(EUCOMM)Hmgu} page
• IMPC: Gatad2b gene page

Mutation origin

• Mutant Cell Lines: HEPD0554_7_C01, HEPD0554_7_C02, HEPD0554_7_C04, HEPD0554_7_E03, HEPD0554_7_E04, HEPD0554_7_F06, HEPD0554_7_G02, HEPD0554_7_H02, HEPD0554_7_H03, HEPD0554_7_H05
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 90255407 of Chromosome 3 upstream of the critical exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 90257058. Exons 2and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. RT-qPCR showed that heterozygotes express ~50% of mRNA relative to wild-type controls. Western blot analysis of E18 cerebella showed ~50% of protein content in heterozygotes and absence of protein expression in homozygotes. Immunostaining of forebrain sections from E16.5 embryos confirmed the reduced and absent expression in the nuclei of cells in the cortex of heterozygous and homozygous embryos, respectively. (J:157065, J:344365)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Gatad2b Mutation: 24 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 3 reference(s)