Igh-J^tm1.1Lave
Targeted Allele Detail

Summary

• Symbol: Igh-J^tm1.1Lave
• Name: immunoglobulin heavy chain, joining region; targeted mutation 1.1, Laurent Verkoczy
• MGI ID: MGI:4421672
• Synonyms: 2f5, 2F5 VH⁺, Igh-J^{tm1.1(2F5)Lave}
• Gene: Igh-J Location: unknown Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155798
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

Allele Type: Targeted Mutations: Insertion, Intergenic deletion Mutation details: The human monoclonal antibody 2F5 was targeted into the J region removing J1 through 4 to produce chimeric human/mouse 2F5 antibodies. A floxed neo cassette used for ES cell selection was removed by cre mediated recombination. The 2F5 antibody is a polyreactive and broadly neutralizing antibody that protects against simian HIV challenge. (J:155798)

Generation of the Igh-J^tm1.1Lave allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh-J Mutation: 13 strains or lines available

References

• Original: J:155798 Verkoczy L, et al., Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance. Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):181-6
• All: 5 reference(s)