Tbx2^{tm1.1(cre)Vmc}
Targeted Allele Detail

Summary

• Symbol: Tbx2^{tm1.1(cre)Vmc}
• Name: T-box 2; targeted mutation 1.1, Vincent M Christoffels
• MGI ID: MGI:4415614
• Synonyms: Tbx2^Cre
• Gene: Tbx2 Location: Chr11:85723441-85732774 bp, + strand Genetic Position: Chr11, 51.34 cM
• Alliance: Tbx2^{tm1.1(cre)Vmc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:155719
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Tbx2^{tm1.1(cre)Vmc} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tbx2 (MGI:98494)

• Mutation details: Briefly, 5.9 kb of upstream and 2.2 kb of downstream Tbx2 129/SvJ genomic fragments were amplified by PCR and ligated to an Frt-flanked Cre-polyA-PGK-neo cassette replacing the first five codons of the Tbx2-coding region in exon 1. A diphteria toxin negative-selection cassette was placed at the 5' end of the targeting construct. Homologous recombination was performed in E14IB10 ES cells. The PGK-neo cassette in Tbx2^CreNEO animals was removed subsequently by crossing with FlpE mice. Tbx2 expression is undetectable in homozygous embryos which show a similar hindlimb phenotype to that observed in Tbx2-deficient (Tbx2^tm1Pa) embryos. (J:155719)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tbx2 (mouse) Summary of all recombinase alleles driven by Tbx2. Alliance mouse Tbx2 gene page

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbx2 Mutation: 27 strains or lines available

References

• Original: J:155719 Aanhaanen WT, et al., The Tbx2+ primary myocardium of the atrioventricular canal forms the atrioventricular node and the base of the left ventricle. Circ Res. 2009 Jun 5;104(11):1267-74
• All: 15 reference(s)