Nipbl^{Gt(EUCE313f02)Hmgu}
Gene trapped Allele Detail

Summary

• Symbol: Nipbl^{Gt(EUCE313f02)Hmgu}
• Name: NIPBL cohesin loading factor; gene trap EUCE313f02, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4374347
• Synonyms: Nipbl^FLEX
• Gene: Nipbl Location: Chr15:8320101-8473947 bp, - strand Genetic Position: Chr15, 3.82 cM, cytoband A2
• Alliance: Nipbl^{Gt(EUCE313f02)Hmgu} page

Mutation origin

• Mutant Cell Line: EUCE313f02
• Germline Transmission: Earliest citation of germline transmission: J:236978
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Conditional ready, Null/knockout)
• Mutation: Insertion of gene trap vector Vector: rsFlipROSAbgeo*
• Mutation details: A conditional-ready gene trap cassette with FRT, F3, loxP, and Lox5171 sites upstream of a beta-geo reporter followed downstream by loxP, lox5171, FRT, and F3 sites in inverse orientation, was inserted into exon 1. This insertion is in the original gene-trapped configuration so that transcription is stopped at the beta-geo reporter, yielding a null allele, which can be excised into a functional allele through Flp- or Cre-mediated excision. (J:236978)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  EUCE313f02.p1ka3SPK	FI566424 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr15:8457849-8458321 bp (-)
  EUCE313f02.q1k5SPK	FI563168 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr15:8458353-8458557 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  56 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nipbl Mutation: 124 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)