Mfsd4a^{tm1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mfsd4a^{tm1(KOMP)Wtsi}
• Name: major facilitator superfamily domain containing 4A; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:4363688
• Gene: Mfsd4a Location: Chr1:131950544-131995800 bp, - strand Genetic Position: Chr1, 57.24 cM
• IMPC: Mfsd4a gene page

Mutation origin

• Mutant Cell Lines: EPD0321_2_A11, EPD0321_2_B09, EPD0321_2_B10, EPD0321_2_C09, EPD0321_2_C11, EPD0321_2_E10, EPD0321_2_G12, EPD0321_2_H11
• Germline Transmission: Unknown
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 7061 starting at position 131980096 and ending at position 131987157 of Chromosome 1 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Mfsd4a Mutation: 22 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)