Map4k2^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Map4k2^{tm1a(KOMP)Wtsi}
• Name: mitogen-activated protein kinase kinase kinase kinase 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4362846
• Gene: Map4k2 Location: Chr19:6391165-6405645 bp, + strand Genetic Position: Chr19, 4.46 cM, cytoband A
• Alliance: Map4k2^{tm1a(KOMP)Wtsi} page
• IMPC: Map4k2 gene page

Mutation origin

• Mutant Cell Lines: EPD0308_2_A03, EPD0308_2_B03, EPD0308_2_C03, EPD0308_2_D04, EPD0308_2_E03, EPD0308_2_E04, EPD0308_2_F01, EPD0308_2_G02, EPD0308_2_G03, EPD0308_2_H03, EPD0308_2_H04
• Germline Transmission: Earliest citation of germline transmission: J:382684
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details:

  The L1L2_Bact_P cassette was inserted at position 6394908 of Chromosome 19 upstream of the critical exon 15 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 24 at position 6397103. The critical exons 15-24 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 2 lines available
• Carrying any Map4k2 Mutation: 42 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)