Rfk^tm1.1Jcbr
Targeted Allele Detail

Summary

• Symbol: Rfk^tm1.1Jcbr
• Name: riboflavin kinase; targeted mutation 1.1, Jens C Bruning
• MGI ID: MGI:4356069
• Synonyms: Rfk^flox
• Gene: Rfk Location: Chr19:17371407-17378713 bp, + strand Genetic Position: Chr19, 12.82 cM, cytoband B
• Alliance: Rfk^tm1.1Jcbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151999
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A frt-flanked neomycin cassette and an upstream loxP site was inserted upstream of exon 1. A second loxP site was inserted downstream of exon 2 at nucleotide position 1080. Founder mice were generated from homologously targeted clones and then crossed with Flp-deleter to remove the neomycin cassette and leave exons 1 and 2 floxed. Correct targeting was confirmed by Southern blotting. (J:151999)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rfk Mutation: 21 strains or lines available

References

• Original: J:151999 Yazdanpanah B, et al., Riboflavin kinase couples TNF receptor 1 to NADPH oxidase. Nature. 2009 Aug 27;460(7259):1159-63
• All: 2 reference(s)