Ifnb1^tm2.1Lien
Targeted Allele Detail

Summary

• Symbol: Ifnb1^tm2.1Lien
• Name: interferon beta 1, fibroblast; targeted mutation 2.1, Stefan Lienenklaus
• MGI ID: MGI:4355683
• Synonyms: IFN-B^{flox beta-luc}, IFN-B^floxbeta-luc
• Gene: Ifnb1 Location: Chr4:88440262-88441011 bp, - strand Genetic Position: Chr4, 41.91 cM
• Alliance: Ifnb1^tm2.1Lien page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151854
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted 42 bp upstream of the lone coding exon and a Frt-flanked neomycin cassette, another loxP site, and a myc-tagged luciferase coding sequence were inserted downstream of the exon in the BstAPI restriction site. After targeting in C57BL/6 ES cells, the neomycin cassette was removed by crossing founder mice with FLP-deleter mice. A poly-A sequence upstream of the luciferase cassette prevents transcription of the reporter until the endogenous exon is floxed. (J:151854)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifnb1 Mutation: 20 strains or lines available

References

• Original: J:151854 Lienenklaus S, et al., Novel reporter mouse reveals constitutive and inflammatory expression of IFN-beta in vivo. J Immunol. 2009 Sep 1;183(5):3229-36
• All: 6 reference(s)