Tpp2^{Gt(OST432061)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Tpp2^{Gt(OST432061)Lex}
• Name: tripeptidyl peptidase II; gene trap OST432061, Lexicon Genetics
• MGI ID: MGI:4315077
• Gene: Tpp2 Location: Chr1:43973130-44042160 bp, + strand Genetic Position: Chr1, 23.5 cM
• Alliance: Tpp2^{Gt(OST432061)Lex} page

Mutation origin

• Mutant Cell Line: OST432061
• Germline Transmission: Earliest citation of germline transmission: J:157195
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Not Applicable)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene-trap cassette containing a splice acceptor and a poly(A) signal was inserted between exons 2 and 3, so that a spliced transcript containing exons 1 and 2 (98 out of 1262 aa) is expressed instead of the full-length transcript. RT-PCR of MEFs showed reduced mRNA expression. Western blotting of spleen, liver, heart and kidney from homozygous mice failed to detect any wild-type protein. (J:157195)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST432061	CG657540 ; (MGI Seq Detail)	3' RACE	Chr1:44009564-44010972 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  13 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpp2 Mutation: 83 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)