Cisd2^{Gt(XS0445)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Cisd2^{Gt(XS0445)Wtsi}
• Name: CDGSH iron sulfur domain 2; gene trap XS0445, Wellcome Trust Sanger Institute
• MGI ID: MGI:3877354
• Gene: Cisd2 Location: Chr3:135112173-135129194 bp, - strand Genetic Position: Chr3, 62.55 cM, cytoband H2
• Alliance: Cisd2^{Gt(XS0445)Wtsi} page

Mutation origin

• Mutant Cell Line: XS0445
• Germline Transmission: Earliest citation of germline transmission: J:183788
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxf
• Mutation details: The gene trap inserted into exon 1 (encoding 34 amino acids and lacking the ER-targeting TM segment), and fused the gene encoding beta-Galactosidase to the endogenous sequence. Analysis of protein expression in mouse spleen tissue extracted from homozygous mice confirmed the absence of the encoded protein expression by immunoblot analysis. (J:183788)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XS0445	DX574833 ; (MGI Seq Detail)	5' RACE	multiple
  * GRCm39; (strand of gene trap mutagenesis)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cisd2 Mutation: 36 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)