Kif13b^{Gt(AF0005)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Kif13b^{Gt(AF0005)Wtsi}
• Name: kinesin family member 13B; gene trap AF0005, Wellcome Trust Sanger Institute
• MGI ID: MGI:3876683
• Synonyms: KIF13BdeltaCG
• Gene: Kif13b Location: Chr14:64889633-65047067 bp, + strand Genetic Position: Chr14, 33.77 cM, cytoband C3
• Alliance: Kif13b^{Gt(AF0005)Wtsi} page

Mutation origin

• Mutant Cell Line: AF0005
• Germline Transmission: Earliest citation of germline transmission: J:282570
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene-trap vector (beta-geo) is inserted downstream of exon 38. The mutation results in the removal of the C-terminus of the gene, including a proline-rich domain and the Cytoskeleton-Associated Protein Glycine-rich (CAP-Gly) domain (amino acids 1521-1843). The expression of truncated protein is not identifiable by Western blotting, but can be identified by LacZ staining of embryos and mouse embryonic fibroblasts. (J:282570)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AF0005	DX574162 ; (MGI Seq Detail)	5' RACE	Chr14:65016799-65027146 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  42 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kif13b Mutation: 109 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)