Trpc5^tm1.1Clph
Targeted Allele Detail

Summary

• Symbol: Trpc5^tm1.1Clph
• Name: transient receptor potential cation channel, subfamily C, member 5; targeted mutation 1.1, David E Clapham
• MGI ID: MGI:3849324
• Synonyms: TRPC5^-, Trpc5-E5^-
• Gene: Trpc5 Location: ChrX:143164667-143471176 bp, - strand Genetic Position: ChrX, 65.49 cM, cytoband F2
• Alliance: Trpc5^tm1.1Clph page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148915
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single loxP site before exon 5 and a PGK-neo cassette flanked by two frt sites followed by a loxP site after exon 5 was introduced into the locus via homologous recombination. The exon 5 genomic region encoding the putative 4th transmembrane domain was deleted in ES cells by transient cre expression. RT-PCR analysis of transcripts from whole brain confirmed the absence of exon 5. Immunoprecipitation and Western blot analyses confirmed loss of protein product. (J:148915)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Trpc5 Mutation: 19 strains or lines available

References

• Original: J:148915 Riccio A, et al., Essential role for TRPC5 in amygdala function and fear-related behavior. Cell. 2009 May 15;137(4):761-72
• All: 24 reference(s)