Pou4f3tm1.1Nat
Targeted Allele Detail
|
Symbol: |
Pou4f3tm1.1Nat |
Name: |
POU domain, class 4, transcription factor 3; targeted mutation 1.1, Jeremy Nathans |
MGI ID: |
MGI:3849065 |
Synonyms: |
Brn3c-CKOAP |
Gene: |
Pou4f3 Location: Chr18:42527662-42529158 bp, + strand Genetic Position: Chr18, 22.58 cM, cytoband B3-E1
|
Alliance: |
Pou4f3tm1.1Nat page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:177259
|
Parent Cell Line: |
Not Specified (ES Cell)
|
Strain of Origin: |
129
|
|
Allele Type: |
|
Targeted (Conditional ready, No functional change, Reporter) |
Mutations: |
|
Insertion, Intragenic deletion
|
|
|
Mutation details: A loxP site was introduced to the 5' UTR (50 bp upstream of the ATG start codon), and three repeats of the SV40 early terminator were placed in the 3' UTR (300 bp downstream of the stop codon) along with a second loxP site, the human placental alkaline phosphatase (AP) gene, and an FRT-flanked neomycin resistance gene. The targeting vector was introduced to 129-derived embryonic stem cells. The neomycin cassette was excised with a germline FLP recombinase.
(J:177259)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
Original: |
J:177259 Badea T, et al., Creation of a conditional knock-in alkaline phosphatase reporter in Pou4f3. MGI Direct Data Submission. 2011; |
All: |
4 reference(s) |
|