Fat4^tm2Hmc
Targeted Allele Detail

Summary

• Symbol: Fat4^tm2Hmc
• Name: FAT atypical cadherin 4; targeted mutation 2, Helen McNeill
• MGI ID: MGI:3846574
• Synonyms: Fat4^5A7
• Gene: Fat4 Location: Chr3:38941089-39066134 bp, + strand Genetic Position: Chr3, 18.72 cM
• Alliance: Fat4^tm2Hmc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:138247
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 3 and a loxP site and frt flanked PGK-neo cassette were inserted downstream of exon 3 via homologous recombination. Two mutant ES cell lines acquired the two loxP insertion in the targeted gene. One allele is derived from line 2E11 in which targeting occured as expected, Fat4^tm1Hmc . Insertion of the targeting vector in the 5A7 line induced a recombination of the Fat4 locus, resulting in this null allele. (J:138247)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fat4 Mutation: 223 strains or lines available

Notes

This mouse is no longer being maintained. However, the 5A7 ES cell line from which it was derived is still being kept by Dr. Helen McNeill.

References

• Original: J:138247 Saburi S, et al., Loss of Fat4 disrupts PCP signaling and oriented cell division and leads to cystic kidney disease. Nat Genet. 2008 Aug;40(8):1010-5
• All: 1 reference(s)