Gt(ROSA)26Sor^{tm1(Nfatc2*)Rao}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Nfatc2*)Rao}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Anjana Rao
• MGI ID: MGI:3844634
• Synonyms: R26^{STOPflox-V-NFAT1}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Nfatc2*)Rao} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148341
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Nfatc2*)Rao} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nfatc2 (MGI:102463)

• Mutation details: A construct consisting of a splice acceptor site, floxed neo-stop cassette, hyperactivatable Nfatc2 gene, and an frt flanked IRES-GFP cassette was inserted into intron 1 via homologous recombination. The hyperactivatable Nfatc2 gene contains mutations in the calcineurin docking site resulting in higher affinity for ligand. The mutant protein sequence is HPVIVITGP. (J:148341)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:148341 Muller MR, et al., Requirement for balanced Ca/NFAT signaling in hematopoietic and embryonic development. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7034-9
• All: 1 reference(s)