Rbx1^Gt(XB674)Byg
Gene trapped Allele Detail

Summary

• Symbol: Rbx1^Gt(XB674)Byg
• Name: ring-box 1; gene trap XB674, BayGenomics
• MGI ID: MGI:3842436
• Gene: Rbx1 Location: Chr15:81350517-81360570 bp, + strand Genetic Position: Chr15, 38.11 cM
• Alliance: Rbx1^Gt(XB674)Byg page

A Rbx1^Gt(XB674)Byg/Rbx1^Gt(XB674)Byg embryo at E6.5 is viable but smaller than wild-type or heterozygous embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: XB674
• Germline Transmission: Earliest citation of germline transmission: J:147759
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: The gene trap vector pGT0pfs was inserted into intron 1 at nucleotide 1186, relative to the translation start site. Immunohistochemical analysis confirmed the absence of protein expression in homozygous embryos. (J:147759)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB674	CC156285 ; (MGI Seq Detail)	5' RACE	multiple
  * GRCm39; (strand of gene trap mutagenesis)

Generation of the Rbx1^Gt(XB674)Byg allele

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbx1 Mutation: 38 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:147759 Tan M, et al., RBX1/ROC1 disruption results in early embryonic lethality due to proliferation failure, partially rescued by simultaneous loss of p27. Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6203-8
• All: 2 reference(s)