Igh^tm3Mnz
Targeted Allele Detail

Summary

• Symbol: Igh^tm3Mnz
• Name: immunoglobulin heavy chain complex; targeted mutation 3, Michel C Nussenzweig
• MGI ID: MGI:3836312
• Synonyms: IgH^I
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145691
• Parent Cell Line: CY2.4 (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

Allele Type: Targeted (Not Applicable) Mutation: Insertion Mutation details: A targeting vector containing a self-excising loxP-flanked neomycin/sperm-specific Cre cassette was used to target a loxP site and a I-SceI endonuclease site upstream of the S-mu switching region and downstream of the E-mu enhancer. The construct was electroporated into albino C57BL/6 embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male founder had self-excision of the neomycin/Cre cassette, leaving the loxP and I-SceI sites in the germline. Correct targeting was confirmed by Southern blotting. B cell development and numbers were normal in heterozygote mice. (J:145691)

Generation of the Igh^tm3Mnz allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:145691 Robbiani DF, et al., AID is required for the chromosomal breaks in c-myc that lead to c-myc/IgH translocations. Cell. 2008 Dec 12;135(6):1028-38
• All: 7 reference(s)