Gli2^tm1Blnw
Targeted Allele Detail

Summary

• Symbol: Gli2^tm1Blnw
• Name: GLI-Kruppel family member GLI2; targeted mutation 1, Baolin Wang
• MGI ID: MGI:3834146
• Synonyms: Glir^P1-4
• Gene: Gli2 Location: Chr1:118761862-118981349 bp, - strand Genetic Position: Chr1, 52.17 cM, cytoband E2-E4
• Alliance: Gli2^tm1Blnw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145182
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin cassette was inserted upstream of the last exon, part of which was replaced with corresponding mouse cDNA sequences containing Ser-Ala mutations in the codons for the first four protein kinase A sites (S789A, S805A, S817A and S848A). The neomycin was left in place because of the increased lethality of heterozygotes that occured when it was removed. (J:145182)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gli2 Mutation: 173 strains or lines available

References

• Original: J:145182 Pan Y, et al., Phosphorylation of Gli2 by protein kinase A is required for Gli2 processing and degradation and the Sonic Hedgehog-regulated mouse development. Dev Biol. 2009 Feb 1;326(1):177-89
• All: 1 reference(s)