Tg(Myh6-Pln)11Egk
Transgene Detail

Summary

• Symbol: Tg(Myh6-Pln)11Egk
• Name: transgene insertion 11, Evangelia G Kranias
• MGI ID: MGI:3833963
• Synonyms: S16A mutant PLB, S16A PLB, Ser¹⁶-->Ala mutant PLB, Tg(Pln)11Egk
• Transgene: Tg(Myh6-Pln)11Egk Location: unknown
• Alliance: Tg(Myh6-Pln)11Egk page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Pln)11Egk expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pln (MGI:97622)

• Mutation details: The transgene contains a mouse cardiac phospholamban cDNA with a point mutation (TCC->GCC) that encodes alanine instead of serine at amino acid position 16 of the protein (S16A), followed by an SV40 polyadenylation signal, under control of the cardiac specific alpha myosin heavy chain (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. The replaced serine normally is phosphorylated in response to stimulation of cardiac muscle beta adrenergic receptors. Northern blot analysis revealed cardiac expression of the 1.0 kb transcript from the transgene, and quantitative immunoblot analysis demonstrated expression of phospholamban protein in the hearts of transgenic mice homozygous for a null phospholamban allele. (J:119619, J:135899)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:119619 Luo W, et al., Transgenic approaches to define the functional role of dual site phospholamban phosphorylation. J Biol Chem. 1998 Feb 20;273(8):4734-9
• All: 4 reference(s)