Ifng^{tm1(Thy1)Weav}
Targeted Allele Detail

Summary

• Symbol: Ifng^{tm1(Thy1)Weav}
• Name: interferon gamma; targeted mutation 1, Casey T Weaver
• MGI ID: MGI:3831296
• Synonyms: IFN-gamma KI, Ifng/Thy1.1
• Gene: Ifng Location: Chr10:118276951-118281797 bp, + strand Genetic Position: Chr10, 66.75 cM
• Alliance: Ifng^{tm1(Thy1)Weav} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144542
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A construct containing an IRES element, coding sequence for a variant of Thy1 (Thy1.1), and a floxed neomycin selection cassette was knocked into the gene locus immediately after the stop codon but before the polyadenylation site. Founder mice were crossed with EIIa-cre transgenic mice to remove the floxed neomycin cassette, leaving behind a single loxP site. The targeting strategy ensured that the knocked in mRNA transcript contained the endogenous 3' untranslated region and the polyadenylation site that are important for regulation of IFN-gamma expression. Co-expression of Thy1 and IFN-gamma were detected in CD4 T cells cultured under Th1-polarizing conditions. In vivo, Thy1 expression by effector T cells was transient and downregulated rapidly in a manner similar to IFN-gamma expression. (J:144542)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifng Mutation: 47 strains or lines available

References

• Original: J:144542 Harrington LE, et al., Memory CD4 T cells emerge from effector T-cell progenitors. Nature. 2008 Mar 20;452(7185):356-60
• All: 16 reference(s)