Smarca4^tm1Tich
Targeted Allele Detail

Summary

• Symbol: Smarca4^tm1Tich
• Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; targeted mutation 1, Tian Chi
• MGI ID: MGI:3830390
• Synonyms: Brg^O
• Gene: Smarca4 Location: Chr9:21527465-21615526 bp, + strand Genetic Position: Chr9, 7.84 cM
• Alliance: Smarca4^tm1Tich page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144072
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with one that contained nucleotide substitutions leading to the amino acid substitution of arginning for lysine at position 785. A floxed gene trap vector with a neo cassette and GFP gene was inserted downstream of exon 2. In the absence of cre, a truncated transcript (of 813 amino acids) with the K785R mutation is expressed, but it is quickly degraded and no protein product is detected by western blot analysis. Removal of the gene trap vector by cre-mediated recombination is required to express a full-length product with the K785R mutation. (J:144072)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smarca4 Mutation: 109 strains or lines available

References

• Original: J:144072 Jani A, et al., A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development. J Exp Med. 2008 Nov 24;205(12):2813-25
• All: 1 reference(s)