Ifng^tm2(CD4*)Lky
Targeted Allele Detail

Summary

• Symbol: Ifng^tm2(CD4*)Lky
• Name: interferon gamma; targeted mutation 2, Richard M Locksley
• MGI ID: MGI:3829361
• Synonyms: DTAG
• Gene: Ifng Location: Chr10:118276951-118281797 bp, + strand Genetic Position: Chr10, 66.75 cM
• Alliance: Ifng^tm2(CD4*)Lky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142395
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Ifng^tm2(CD4*)Lky expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CD4 (920)

  Cd4	(MGI:88335)

  mouse

  Ifng (MGI:107656)
• Mutation details: The following was inserted at the translation start site: a destabilized enhanced cyan fluorescent protein (d2ECFP), a viral self-cleaning 2A sequence, 4 introns and exons of the Ifng gene, a viral IRES, a mutated non-signaling human CD4 cDNA and an endogenous polyadenylation site. Expression at this locus produces ECFP, the Ifng transcript and a non-signaling human CD4. (J:142395)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifng Mutation: 47 strains or lines available

Notes

DTAG = Dual Targeted At IFN-Gamma locus

References

• Original: J:142395 Kang SJ, et al., Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. Immunity. 2008 Nov;29(5):819-33
• All: 1 reference(s)