Apc^tm1Tyj
Targeted Allele Detail

Summary

• Symbol: Apc^tm1Tyj
• Name: APC, WNT signaling pathway regulator; targeted mutation 1, Tyler Jacks
• MGI ID: MGI:3829069
• Synonyms: APC^fle1-15
• Gene: Apc Location: Chr18:34353977-34455605 bp, + strand Genetic Position: Chr18, 18.53 cM
• Alliance: Apc^tm1Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:159014
• Parent Cell Line: V26.2 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. (J:159014)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Apc^tm1Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Apc Mutation: 154 strains or lines available

References

• Original: J:159014 Jacks T, Generation of a conditional Apc allele. MGI Direct Data Submission. 2010
• All: 29 reference(s)