Raf1^tm3Ulb
Targeted Allele Detail

Summary

• Symbol: Raf1^tm3Ulb
• Name: v-raf-leukemia viral oncogene 1; targeted mutation 3, Catrin Pritchard
• MGI ID: MGI:3817448
• Synonyms: Craf^D486A, craf^DA
• Gene: Raf1 Location: Chr6:115595530-115653596 bp, - strand Genetic Position: Chr6, 53.62 cM, cytoband C3
• Alliance: Raf1^tm3Ulb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:141095
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A floxed neo cassette was inserted in intron 13 and a D486A mutation was inserted in exon 14 via homologous recombination. The neo cassette was subsequently removed via in vivo Cre mediated recombination. The D486A mutation creates a kinase inactive protein. Western blot analysis detected a greater than 50 fold reduction in protein expression in homozygous embryos and MEFs. (J:141095)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Raf1 Mutation: 116 strains or lines available

References

• Original: J:141095 Noble C, et al., CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation. Mol Cell. 2008 Sep 26;31(6):862-72
• All: 2 reference(s)