Wnt9b^tm1.2Amc
Targeted Allele Detail

Summary

• Symbol: Wnt9b^tm1.2Amc
• Name: wingless-type MMTV integration site family, member 9B; targeted mutation 1.2, Andrew P McMahon
• MGI ID: MGI:3805020
• Synonyms: Wnt9b^c, Wnt9b^flox
• Gene: Wnt9b Location: Chr11:103618190-103640647 bp, - strand Genetic Position: Chr11, 67.47 cM
• Alliance: Wnt9b^tm1.2Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100575
• Parent Cell Line: AV3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a loxP site just upstream of exon 2, and a frt-flanked PGK-Neo cassette followed by a second loxP site just downstream of exon 2 of the targeted gene to generate Wnt9bM^tm1Amc mice (Wnt9b^cneo. To remove the frt-flanked selection cassette, these mice were then bred to an FLPe-expressing strain (on the Swiss-Webster genetic background). The resulting Wnt9bc offspring carry a loxP site just upstream of exon 2, and a single remaining frt-site followed by a second loxP site just downstream of exon 2. (J:100575)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wnt9b Mutation: 22 strains or lines available

References

• Original: J:100575 Carroll TJ, et al., Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. Dev Cell. 2005 Aug;9(2):283-92
• All: 6 reference(s)