Arid1a^tm1.2Zhwa
Targeted Allele Detail

Summary

• Symbol: Arid1a^tm1.2Zhwa
• Name: AT-rich interaction domain 1A; targeted mutation 1.2, Zhong Wang
• MGI ID: MGI:3796520
• Synonyms: BAF250a^-
• Gene: Arid1a Location: Chr4:133406319-133484080 bp, - strand Genetic Position: Chr4, 66.25 cM
• Alliance: Arid1a^tm1.2Zhwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134633
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: An frt-flanked betageo cassette with a 3' loxP site and a 5' SV40-polyA tail was inserted downstream of exon 8 and an additional loxP site was inserted upstream of exon 8. Transient flp expression in ES cells was used to remove the betageo cassette and leave exon 8 floxed. Cre expression in germline cells removed exon 8 and created a null allele. No gene product was detected by PCR in homozygous null embryos. (J:134633)

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arid1a Mutation: 181 strains or lines available

References

• Original: J:134633 Gao X, et al., ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a. Proc Natl Acad Sci U S A. 2008 May 6;105(18):6656-61
• All: 1 reference(s)