Klf5^Gt(XB751)Byg
Gene trapped Allele Detail

Summary

• Symbol: Klf5^Gt(XB751)Byg
• Name: Kruppel-like transcription factor 5; gene trap XB751, BayGenomics
• MGI ID: MGI:3796190
• Synonyms: KB, Klf5^-, Klf^Betageo
• Gene: Klf5 Location: Chr14:99536127-99550848 bp, + strand Genetic Position: Chr14, 49.43 cM, cytoband E2.1
• Alliance: Klf5^Gt(XB751)Byg page

Mutation origin

• Mutant Cell Line: XB751
• Germline Transmission: Earliest citation of germline transmission: J:136125
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: The gene trap vector containing a beta-geo cassette was inserted into the first intron. The reduction of protein product was confirmed by western blot analysis and immunohistochemistry of heterozygous tissues. This is a gene trap allele, a reporter allele and a hypomorphic allele. (J:136125)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB751	CC156251 ; (MGI Seq Detail)	5' RACE	Chr14:99536593-99536653 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  26 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klf5 Mutation: 38 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:136125 McConnell BB, et al., Kruppel-like factor 5 mediates transmissible murine colonic hyperplasia caused by Citrobacter rodentium infection. Gastroenterology. 2008 Apr;134(4):1007-16
• All: 4 reference(s)