Cfp^tm1Song
Targeted Allele Detail

Summary

• Symbol: Cfp^tm1Song
• Name: complement factor properdin; targeted mutation 1, Wen-Chao Song
• MGI ID: MGI:3771610
• Synonyms: properdin^-
• Gene: Cfp Location: ChrX:20791693-20797794 bp, - strand Genetic Position: ChrX, 16.44 cM, cytoband A3
• Alliance: Cfp^tm1Song page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130108
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was constructed where a loxP site was inserted upstream of exon 3, and a FRT flanked neomycin resistance cassette followed by a loxP site was inserted downstream of exon 5. Upon recombination, the neomycin cassette inserted in the intended location but the upstream loxP site failed to recombine. Analysis of chimeric mice revealed that the insertion of the neomycin cassette was sufficient to prevent transcription of the targeted gene. Gene inactivation was confirmed by failure to detect the gene product in the sera of homozygotes by an immunodiffusion assay. (J:130108)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfp Mutation: 3 strains or lines available

References

• Original: J:130108 Kimura Y, et al., Activator-specific requirement of properdin in the initiation and amplification of the alternative pathway complement. Blood. 2008 Jan 15;111(2):732-40
• All: 9 reference(s)