Epo^{Tg(TAg/Epo)134.3LCPjr}
Transgenic Allele Detail

Summary

• Symbol: Epo^{Tg(TAg/Epo)134.3LCPjr}
• Name: erythropoietin; transgene insertion 134.3 LC, Peter J Ratcliffe
• MGI ID: MGI:3767166
• Synonyms: EpoTAg^-, Epo-Tag^h, Epo^{Tg(SV40T/Epo)134.3LCPjr}, TgH(eposvT)1Pjr
• Gene: Epo Location: Chr5:137481282-137484078 bp, - strand Genetic Position: Chr5, 76.5 cM, cytoband G
• Alliance: Epo^{Tg(TAg/Epo)134.3LCPjr} page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Epo^{Tg(TAg/Epo)134.3LCPjr} expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Epo (MGI:95407)	Transgene codes for a fusion protein of SV40 large T antigen and mouse Epo.

• Mutation details: This transgene contains the wild-type allele of the SV40 large T antigen (TAg) gene fused to the mouse Epo gene isolated from a BALB/c library. In this founder line, a single copy of the transgene replaced one copy of the endogenous Epo gene. This recombination event was confirmed by restriction digest mapping and Southern blotting. Sv40 T antigen was detected in the interstitial cells of the kidney. Epo transgene expression in the kidney is reduced compared to endogenous Epo gene expression as determined by RNAse protection assay.

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Epo Mutation: 32 strains or lines available

References

• Original: J:128415 Maxwell PH, et al., Identification of the renal erythropoietin-producing cells using transgenic mice. Kidney Int. 1993 Nov;44(5):1149-62
• All: 10 reference(s)