Grem1^tm1Ecan
Targeted Allele Detail

Summary

• Symbol: Grem1^tm1Ecan
• Name: gremlin 1, DAN family BMP antagonist; targeted mutation 1, Ernesto Canalis
• MGI ID: MGI:3761925
• Synonyms: Grem1^flox, grem1^loxP
• Gene: Grem1 Location: Chr2:113579020-113588993 bp, - strand Genetic Position: Chr2, 57.43 cM
• Alliance: Grem1^tm1Ecan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126375
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129 x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was placed 335 bp upstream of exon 2. A second loxP site along with a Frt-flanked PGK-neomycin selection cassette was placed within exon 2, 550 bp downstream of the stop codon and deleting the final 66 bp of the exon. Expression of the targeted locus was unchanged in homozygous mice as determined by quantitative PCR. (J:126375)

Expression

In Mice Carrying this Mutation:	20 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grem1 Mutation: 16 strains or lines available

References

• Original: J:126375 Gazzerro E, et al., Conditional deletion of gremlin causes a transient increase in bone formation and bone mass. J Biol Chem. 2007 Oct 26;282(43):31549-57
• All: 12 reference(s)