Rapgef2^tm1.1Tkat
Targeted Allele Detail

Summary

• Symbol: Rapgef2^tm1.1Tkat
• Name: Rap guanine nucleotide exchange factor (GEF) 2; targeted mutation 1.1, Tohru Kataoka
• MGI ID: MGI:3760243
• Synonyms: RA-GEF-1^-
• Gene: Rapgef2 Location: Chr3:78969823-79193824 bp, - strand Genetic Position: Chr3, 34.91 cM
• Alliance: Rapgef2^tm1.1Tkat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125199
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 15 was removed by cre-mediated recombination following a cross with Tg(ACTB-cre)13Miya mice. Removal of the exon was confirmed by PCR and absence of a protein product was confirmed by western blot analysis on proteins extracted from E10.5 embryos. (J:125199)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rapgef2 Mutation: 82 strains or lines available

Notes

ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:125199 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

References

• Original: J:125199 Wei P, et al., Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1. Biochem Biophys Res Commun. 2007 Nov 9;363(1):106-12
• All: 2 reference(s)