Il7r^tm1Abra
Targeted Allele Detail

Summary

• Symbol: Il7r^tm1Abra
• Name: interleukin 7 receptor; targeted mutation 1, Ninan Abraham
• MGI ID: MGI:3758859
• Synonyms: IL-7Ralpha^449F
• Gene: Il7r Location: Chr15:9505874-9530262 bp, - strand Genetic Position: Chr15, 4.16 cM
• Alliance: Il7r^tm1Abra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125360
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted
• Mutation: Other
• Mutation details: Exons 6 through 8 were replaced with a cDNA encoding exons 6 through 8 with a modification in exon 8 that creates a tyrosine to phenylalanine substitution at amino acid position 449. This substitution disrupts the YXXM motif that when phosphorylated binds to the Src homology 2 domain of PI3 kinase to activate downstream pathways. A floxed neo cassette inserted downstream of exon 8 was removed by cre-mediated recombination. Expression and subcellular localization, as determined by western blot and flow cytometry, are not altered. Downstream signaling through Erk and p38 MAPK are unaffected whereas signaling via STAT5 is affected. (J:125360)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il7r Mutation: 29 strains or lines available

Notes

Knock in creates a Y449F substitution.

References

• Original: J:125360 Osborne LC, et al., Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice. J Exp Med. 2007 Mar 19;204(3):619-31
• All: 7 reference(s)