Cd28^tm1Jmg
Targeted Allele Detail

Summary

• Symbol: Cd28^tm1Jmg
• Name: CD28 antigen; targeted mutation 1, Jonathan M Green
• MGI ID: MGI:3722546
• Synonyms: CD28-AYAA
• Gene: Cd28 Location: Chr1:60785547-60812521 bp, + strand Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
• Alliance: Cd28^tm1Jmg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124570
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A targeting vector was designed to insert alanine substitutions for prolines 187 and 190 in exon 4 (P187A, P190A), thereby disrupting the motif responsible for interaction with SH3 domain-containing proteins. A floxed neo was included with the vector and subsequently removed via cre mediated recombination. (J:124570)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd28 Mutation: 53 strains or lines available

References

• Original: J:124570 Friend LD, et al., A dose-dependent requirement for the proline motif of CD28 in cellular and humoral immunity revealed by a targeted knockin mutant. J Exp Med. 2006 Sep 4;203(9):2121-33
• All: 12 reference(s)