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Targeted Allele Detail
Symbol: Il4ratm2Tch
Name: interleukin 4 receptor, alpha; targeted mutation 2, Talal A Chatila
MGI ID: MGI:3720593
Synonyms: IL-4RalphaR576, Il4raR576, Q576R
Gene: Il4ra  Location: Chr7:125151443-125178646 bp, + strand  Genetic Position: Chr7, 68.94 cM
Alliance: Il4ratm2Tch page
Lung histology staining of OVA-sensitized control (Q576) and Il4ratm2Tch/Il4ratm2Tch (R576) mice challenged intranasally with OVA on three consecutive days

Show the 4 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:152376
Parent Cell Line:  RW-4 (ES Cell)
Strain of Origin:  129X1/SvJ
Allele Type:    Targeted
Mutation:    Insertion
Mutation detailsThe targeting vector was designed (by site-directed mutagenesis) to change the alanine-guanine to guanine-alanine at basepair 1964-65 of murine IL-4Ra cDNA, corresponding to a single amino acid substitution of glutamine to arginine at codon 576 (Q576R). This also inserted a loxp-flanked PGK-neo cassette within intron 11. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre-expressing vector to remove the selection cassette (leaving a single loxp site in intron 11) and then injected into C57BL/6 blastocysts. (J:152376)
Generation of the Il4ratm2Tch allele
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 3 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Il4ra Mutation:  41 strains or lines available
Original:  J:152376 Tachdjian R, et al., Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma. J Exp Med. 2009 Sep 28;206(10):2191-204
All:  4 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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MGI 6.13
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