Tg(APP-App*R609D*K612E)7Vln
Transgene Detail

Summary

• Symbol: Tg(APP-App*R609D*K612E)7Vln
• Name: transgene insertion 7, Fred Van Leuven
• MGI ID: MGI:3720083
• Synonyms: ae/RK/F/7
• Transgene: Tg(APP-App*R609D*K612E)7Vln Location: unknown
• Alliance: Tg(APP-App*R609D*K612E)7Vln page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(APP-App*R609D*K612E)7Vln expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	App (MGI:88059)	Transgene codes for a protein with arginine to aspartic acid and lysine to glutamic acid mutations.

• Mutation details: Murine App cDNA containing R609D and K612E mutations was inserted downstream of the human APP gene promoter with a beta-globin intron interspersed. The construct was microinjected into 1.5-day-old pre-nuclear embryos from FVB females. Transgene expression in the brain was verified by RT-PCR. Expression was low compared to endogenous App levels in the mouse brain. (J:32213)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:32213 Moechars D, et al., Expression in brain of amyloid precursor protein mutated in the alpha-secretase site causes disturbed behavior, neuronal degeneration and premature death in transgenic mice. EMBO J. 1996 Mar 15;15(6):1265-74
• All: 1 reference(s)