Del(16Dgcr2-Arvcf)1Ais
Targeted Allele Detail

Summary

• Symbol: Del(16Dgcr2-Arvcf)1Ais
• Name: deletion, Chr 16, Arthur I Skoultchi 1
• MGI ID: MGI:3702630
• Synonyms: Del1Ais
• Gene: Del(16Dgcr2-Arvcf)1Ais Location: unknown Genetic Position: Chr16, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64380
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted
• Mutations: Intergenic deletion, Intragenic deletion
• Del(16Dgcr2-Arvcf)1Ais involves 28 genes/genome features (Gm20518, Dgcr2, Tssk1 ...) View all
• Mutation details: These mice were created by either breeding, or by targeting ES cells as follows. A targeting vector containing a PGK promoter, a loxP site and a neomycin resistance gene was inserted into the DiGeorge syndrome critical region gene 2, resulting in the loss of exons 4 and 5. These ES cells were additionally targeted with a vector containing two regions of the armadillo repeat gene deleted in velo-cardio-facial syndrome flanking a hygromycin resistance gene, a loxP site and a promoterless puromycin resistance gene. The insertion of this vector resulted in the loss of amino acids 83-276. Subsequent transient cre expression when the loxP sites were in cis resulted in the deletion of sequence between the two loxP sites. This 550-kb deletion encompasses 16 genes. (J:64380)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(16Dgcr2-Arvcf)1Ais Mutation: 0 strains or lines available

References

• Original: J:64380 Puech A, et al., Normal cardiovascular development in mice deficient for 16 genes in 550 kb of the velocardiofacial/DiGeorge syndrome region. Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10090-5
• All: 1 reference(s)