Wnt1^tm1Amc
Targeted Allele Detail

Summary

• Symbol: Wnt1^tm1Amc
• Name: wingless-type MMTV integration site family, member 1; targeted mutation 1, Andrew P McMahon
• MGI ID: MGI:3700020
• Synonyms: Wnt-1^Enh-
• Gene: Wnt1 Location: Chr15:98687738-98691711 bp, + strand Genetic Position: Chr15, 54.65 cM, cytoband F1-F3
• Alliance: Wnt1^tm1Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:118582
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 5.5 kb enhancer element, located 3' to the Wnt1 polyadenylation signal sequence, was replaced with a floxed PGKneo selection cassette by homologous recombination. This cassette was removed by cre recombination via injection of a cre-encoding plasmid into pronuclei of zygotes from heterozygous intercrosses. (J:118582)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wnt1 Mutation: 30 strains or lines available

References

• Original: J:118582 Danielian PS, et al., A 5.5-kb enhancer is both necessary and sufficient for regulation of Wnt-1 transcription in vivo. Dev Biol. 1997 Dec 15;192(2):300-9
• All: 2 reference(s)