Tg(PSEN1H163R)G9Btla
Transgene Detail

Summary

• Symbol: Tg(PSEN1H163R)G9Btla
• Name: transgene insertion G9, Bruce Lamb
• MGI ID: MGI:3693364
• Synonyms: H163R mutant PS-1 YAC, H163R mutant PS-1 YAC transgenic mouse (line G9), (H163R) PS-1 YAC, PS-1 YAC transgenic line G9
• Transgene: Tg(PSEN1H163R)G9Btla Location: unknown
• Alliance: Tg(PSEN1H163R)G9Btla page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:58050
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(PSEN1H163R)G9Btla expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PSEN1 (5663)

  Psen1

  (MGI:1202717)

• Mutation details: A 1000 kb YAC transgene (788H12) containing the entire human presenilin-1 gene was altered to include the Familial Alzheimer's Disease- (FAD-) associated histidine-to-arginine substitution at amino acid position 163 (H163R). The transgene also includes approximately 550 kb of upstream and 350 kb of downstream flanking DNA. Semi-quantitative RT-PCR of multiple tissues shows expression of H163R mutant human mRNA at 50-70% of the level of wild-type mouse transcripts. Transgenic tissues express a wide spectrum of human presenilin-1 mRNA and protein species; alternatively spliced human mRNAs encoding presenilin 1, with or without the tetrapeptide VRSQ, are detected, and western blot analysis reveals accumulation of an 18-kDa C-terminal peptide. (J:58050)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The transgene construct was injected into embryonic stem (ES) cells.

References

• Original: J:58050 Lamb BA, et al., Amyloid production and deposition in mutant amyloid precursor protein and presenilin-1 yeast artificial chromosome transgenic mice. Nat Neurosci. 1999 Aug;2(8):695-7
• All: 1 reference(s)