Pdx1^tm1Egs
Targeted Allele Detail

Summary

• Symbol: Pdx1^tm1Egs
• Name: pancreatic and duodenal homeobox 1; targeted mutation 1, Edouard G Stanley
• MGI ID: MGI:3688420
• Synonyms: Pdx1^GFP, Pdx1^GFP/W
• Gene: Pdx1 Location: Chr5:147206907-147212658 bp, + strand Genetic Position: Chr5, 86.84 cM
• Alliance: Pdx1^tm1Egs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110137
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector inserted GFP coding sequences and an frt-flanked hygromycin resistance gene into exon 1. Transient transfection with a vector encoding flp recombinase removed the hygromycin resistance gene and one of the frt sites from targeted ES cells. The expression pattern of GFP in homozygous mutant embryos and adult mice mirrors that of the endogenous gene. (J:114151)

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdx1 Mutation: 35 strains or lines available

References

• Original: J:110137 Holland AM, et al., A mouse carrying the green fluorescent protein gene targeted to the Pdx1 locus facilitates the study of pancreas development and function. Genesis. 2006 Jun;44(6):304-7
• All: 7 reference(s)