Kitl^tm1Pbes
Targeted Allele Detail

Summary

• Symbol: Kitl^tm1Pbes
• Name: kit ligand; targeted mutation 1, Peter Besmer
• MGI ID: MGI:3664559
• Synonyms: Sl^KL2
• Gene: Kitl Location: Chr10:99851492-99936278 bp, + strand Genetic Position: Chr10, 51.4 cM
• Alliance: Kitl^tm1Pbes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50150
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 6 was replaced with a neomycin selection cassette. This alternatively spliced exon 6 encodes the major proteolytic cleavage site for the generation of the soluble encoded protein. Homozygous mice express only a transcript that encodes the transmembrane bound form. However, reduced levels of a soluble form of the encoded protein is found in serum of homozygous mice, suggesting that a secondary proteolytic cleavage site is used. (J:50150)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kitl Mutation: 92 strains or lines available

References

• Original: J:50150 Tajima Y, et al., Consequences of exclusive expression in vivo of Kit-ligand lacking the major proteolytic cleavage site. Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11903-8
• All: 1 reference(s)