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Targeted Allele Detail
Symbol: Casttm1Tcs
Name: calpastatin; targeted mutation 1, Takaomi C Saido
MGI ID: MGI:3641275
Gene: Cast  Location: Chr13:74840487-74956929 bp, - strand  Genetic Position: Chr13, 40.52 cM, cytoband C1
Alliance: Casttm1Tcs page
Germline Transmission:  Earliest citation of germline transmission: J:109976
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsMost of exon 6 was replaced with a pgk-neo in reverse orientation. Northern and Western blot analysis confirmed recombination of the locus, as did RT-PCR. Calpastatin activity assays indicated that calpastatin activity was reduced to an undetectable level in the mutant mouse brain. (J:109976)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cast Mutation:  50 strains or lines available
Original:  J:109976 Takano J, et al., Calpain mediates excitotoxic DNA fragmentation via mitochondrial pathways in adult brains: evidence from calpastatin mutant mice. J Biol Chem. 2005 Apr 22;280(16):16175-84
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
The Jackson Laboratory