Ntrk2^tm1.1Tes
Targeted Allele Detail

Summary

• Symbol: Ntrk2^tm1.1Tes
• Name: neurotrophic tyrosine kinase, receptor, type 2; targeted mutation 1.1, Lino Tessarollo
• MGI ID: MGI:3640817
• Synonyms: TrkB^-, TrkB.T1-
• Gene: Ntrk2 Location: Chr13:58954383-59281784 bp, + strand Genetic Position: Chr13, 31.2 cM
• Alliance: Ntrk2^tm1.1Tes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:110478
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: The exon after exon 11 (i.e. exon T1) is used in vivo to produce a dominant negative form of the gene product that has extracellular and transmembrane domains but lacks the intracellular signaling domain. A loxP site was inserted into the BamHI site upstream of exon T1 and a loxP- and frt- flanked neomycin cassette was inserted into BamHI site downstream of exon T1. Cre-mediated recombination was used in correctly targeted mice to remove the T1 exon and the neomycin cassette. No T1 isoform was detected by immunoblot assays of homozygote brain extracts while expression of the full length protein was unaffected. (J:110478)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ntrk2 Mutation: 66 strains or lines available

References

• Original: J:110478 Dorsey SG, et al., In vivo restoration of physiological levels of truncated TrkB.T1 receptor rescues neuronal cell death in a trisomic mouse model. Neuron. 2006 Jul 6;51(1):21-8
• All: 17 reference(s)